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To generate non-transgenic slmlo1 tomato lines, we segregated away the T-DNA by selfing T0 transformant 8 and cultivating next generation (T1) plants. In the progeny, we identified five slmlo1 T-DNA-free individuals (Fig. 1f). Disease resistance assays with the powdery mildew fungus Oidium neolycopersici revealed that all the slmlo1 mutant plants were fully resistant to the pathogen, whereas all tested wild-type plants were susceptible (Fig. 1e). The slmlo1 mutant plants were not only morphologically similar to the wild type (Supplementary Fig. 1), but they also produced harvested fruit weight similar to the wild type (Supplementary Fig. 2). 041b061a72